Manchester Centre for Biophysics and Catalysis (MCBC)

Electron paramagnetic resonance (EPR)

alternative text

Electron paramagnetic resonance (EPR) or electron spin resonance (ESR) spectroscopy studies molecular species that contain unpaired electrons (radicals or transition metals). As "ordinary" molecules do not give rise to any signal, EPR is a technique of great specificity. Biological EPR has recently started contributing to structural biology, through the use spin-labelled species combined with spin distance measurements. In the MIB we have recently established a continuous wave and pulsed EPR/ENDOR/ELDOR facility based around a Bruker ELEXSYS E580 spectrometer operating at X-band (~9.5GHz). Samples can be studied at temperatures from ‘room’ temperature down to liquid helium temperature (4.2K). A laser can be synchronised with the pulsed spectrometer to allow for in situ photo-generation of paramagnetic species. We have also installed dedicated equipment to allow time-resolved EPR using flash-cooled and rapid quench samples.