Manchester Centre for Biophysics and Catalysis (MCBC)

Nuclear magnetic resonance

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The NMR Facility consists of a DPX400MHz instrument, set up for open - access and automation, and a 600MHz research instrument. Pulsed Fourier Transform Nuclear Magnetic Resonance spectroscopy (FT-NMR) is one of the most powerful and versatile non-destructive spectroscopic methods for structure elucidation.

Analysis of a 1-dimensional (1D) NMR spectrum provides information on the number and type of protons and carbon atoms in a molecule. Further pulse programmes such as COSY can reveal their bonding connectivity and orientation. A very common application is NOESY (nuclear Overhauser effect spectroscopy), an NMR technique used to display “through space” connectivity often used to aid in the structural determination of macromolecules i.e. proteins.

The Centre has recently installed high pressure NMR to enable structural analysis at variable pressures and is also expanding to higher field instrumentation in the near future.