Manchester Centre for Biophysics and Catalysis (MCBC)

X-ray crystallography facilities

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The X-ray crystallography facility comprises a suite of dedicated rooms. It provides support in macromolecular crystallisation and diffraction data acquisition. A crystallisation and data collection service is run for those in need of atomic structures but not familiar with the technique. The facility contains a Microfocus rotating anode generator (Bruker MicroStar), providing a high brilliance (2.7kW) source with tuned focussing optics that develop a tightly focussed, highly monochromatic X-ray beam. Diffraction data is acquired via CCD technology. Cryogenic data collection (at ca. 100K) is enabled by a cold stream generator. A Kappa geometry four-circle goniometer enables efficient coverage of diffraction space. The facility includes a number of temperature controlled incubators, a Molecular Dimensions Mosquito and an Art Robbins Phoenix robotic system capable of nanolitre dispensing and a CrysCam automatic plate scanning unit. The facility also provide the ability for dedictated anaerobic crystallography as well as microspectrophotometry capable for recording crystal UV-VIS spectra.

In addition, the facility includes a dedicated laboratory space containing a number of temperature controlled incubators, a Molecular Dimensions Mosquito and an Art Robbins Phoenix robotic system capable of nanolitre dispensing as well as a CrysCam automatic plate scanning unit. The facility also provide the ability for dedictated anaerobic crystallography as well as microspectrophotometry capable for recording crystal UV-VIS spectra.